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human derived osteoblast  (ATCC)


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    Structured Review

    ATCC human derived osteoblast
    Human Derived Osteoblast, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+derived+osteoblast/HOS/pm41621952-304-41-46
    Average 96 stars, based on 1087 article reviews
    human derived osteoblast - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transactivation Assay:

    Article Title: Comprehensive Synthesis of Side-Chain Fluorinated 2α-[2-(Tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D 3 Analogs and Preliminary Biological Activities.
    Article Snippet: .. Transactivation Assay of Human Osteocalcin Promoter A mixed solution of expression plasmids, pGL4.26 DR3 (×4) and pcDNA3-hVDR (full length) at a ratio of 5 : 1, and the control plasmid pGL4-CMV-Rluc (Promega) for correcting transfection efficiency was simultaneously used to transfect human-derived osteoblast-like cells (HOS cells; ATCC, Manassas, VA, U.S.A.) using the gene introduction device MaxCyte STX (MaxCyte, Rockville, Maryland, U.S.A.), so that the final amount of DNA was 300 μg/mL. ..

    Expressing:

    Article Title: Comprehensive Synthesis of Side-Chain Fluorinated 2α-[2-(Tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D 3 Analogs and Preliminary Biological Activities.
    Article Snippet: .. Transactivation Assay of Human Osteocalcin Promoter A mixed solution of expression plasmids, pGL4.26 DR3 (×4) and pcDNA3-hVDR (full length) at a ratio of 5 : 1, and the control plasmid pGL4-CMV-Rluc (Promega) for correcting transfection efficiency was simultaneously used to transfect human-derived osteoblast-like cells (HOS cells; ATCC, Manassas, VA, U.S.A.) using the gene introduction device MaxCyte STX (MaxCyte, Rockville, Maryland, U.S.A.), so that the final amount of DNA was 300 μg/mL. ..

    Control:

    Article Title: Comprehensive Synthesis of Side-Chain Fluorinated 2α-[2-(Tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D 3 Analogs and Preliminary Biological Activities.
    Article Snippet: .. Transactivation Assay of Human Osteocalcin Promoter A mixed solution of expression plasmids, pGL4.26 DR3 (×4) and pcDNA3-hVDR (full length) at a ratio of 5 : 1, and the control plasmid pGL4-CMV-Rluc (Promega) for correcting transfection efficiency was simultaneously used to transfect human-derived osteoblast-like cells (HOS cells; ATCC, Manassas, VA, U.S.A.) using the gene introduction device MaxCyte STX (MaxCyte, Rockville, Maryland, U.S.A.), so that the final amount of DNA was 300 μg/mL. ..

    Plasmid Preparation:

    Article Title: Comprehensive Synthesis of Side-Chain Fluorinated 2α-[2-(Tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D 3 Analogs and Preliminary Biological Activities.
    Article Snippet: .. Transactivation Assay of Human Osteocalcin Promoter A mixed solution of expression plasmids, pGL4.26 DR3 (×4) and pcDNA3-hVDR (full length) at a ratio of 5 : 1, and the control plasmid pGL4-CMV-Rluc (Promega) for correcting transfection efficiency was simultaneously used to transfect human-derived osteoblast-like cells (HOS cells; ATCC, Manassas, VA, U.S.A.) using the gene introduction device MaxCyte STX (MaxCyte, Rockville, Maryland, U.S.A.), so that the final amount of DNA was 300 μg/mL. ..

    Transfection:

    Article Title: Comprehensive Synthesis of Side-Chain Fluorinated 2α-[2-(Tetrazol-2-yl)ethyl]-1α,25-dihydroxyvitamin D 3 Analogs and Preliminary Biological Activities.
    Article Snippet: .. Transactivation Assay of Human Osteocalcin Promoter A mixed solution of expression plasmids, pGL4.26 DR3 (×4) and pcDNA3-hVDR (full length) at a ratio of 5 : 1, and the control plasmid pGL4-CMV-Rluc (Promega) for correcting transfection efficiency was simultaneously used to transfect human-derived osteoblast-like cells (HOS cells; ATCC, Manassas, VA, U.S.A.) using the gene introduction device MaxCyte STX (MaxCyte, Rockville, Maryland, U.S.A.), so that the final amount of DNA was 300 μg/mL. ..



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